이범천 Bum Chun Lee , 심관섭 Gwan Sub Sim , 김진희 Jin Hui Kim , 김진화 Jin Hwa Kim , 표형배 Hyeong Bae Pyo

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Abstract

Selaginella tamariscina with the popular Korean name Keoun Back, is a traditional medicinal plant for therapy of advanced cancer patients in the Orient. In this study, we evaluated anti-aging activity of S. tamariscina using processed technology and investigated diverse biological activities of processed S. tamariscina (PST) as an anti-aging ingredient of cosmetics. PST, heated with sand, used to different purpose compared with origin in medicine. PST raises total phenol concentration and enhances the DPPH radical scavenging activity. For testing intracellular ROS scavenging activity, the cultured human dermal fibroblasts were analyzed by increase in dichlorofluorescein (DCF) fluorescence upon exposure to UVB 20 mJ/cm² after treatment of PST. UVA-induced MMP-1 expression in human dermal fibroblasts was reduced in a dose-dependent manner by PST. Taken together, 4hese results suggest that PST may act as an anti-aging agent by preventing the skin cell from damage induced by UV irradiation, and imply that PST may be useful as a new ingredient for anti-aging cosmetics.

Key Words

Selaginella tamariscina, anti-aging, ROS, antioxidant activity, MMP

심관섭 , 김진화 , 김진희 , 이범천 , 표형배

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Abstract

In this study. we evaluated anti-aging activity of medical plants that protect the skin cell damage induced by UV irradiation. We have investigated diverse biological activities of Selaginella tamariscina as an anti-aging ingredient of cosmetics. S. tamariscina was found to show scavenging activities of radicals and reactive oxygen species (ROS) with the IC₅₀ values of 65.1μg/mL against 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical and 40.9 μg/mL against superoxide radicals in the xanthine/xanthine oxidase system, respectively. For testing intracellular ROS scavenging activity, the cultured human dermal fibroblasts were analyzed by increase in dichlorofluorescein (DCF) fluorescence upon exposure to UVB 20 mJ/cm² after treatment of S. tamariscina. UVA-induced MMP-1 protein and mRNA expression in human dermal fibroblasts were reduced in a dose-dependent manner by S. tamariscina. Moreover, S. tamariscina inhibited MMP-2 (gelatinase) activity in UVA-irradiated human dermal fibroblasts assayed by zymography and semi-quantitative RT-PCR. Taken together, these results suggest that S. tamariscina may act as an anti-aging agent by Increasing collagen and preventing the skin cell damage induced by UV irradiation, and imply that S. tamariscina nay be useful as a new ingredient for anti-aging cosmetics.

Key Words

Selaginella tamariscina, anti-aging, ROS, antioxidant activity, MMP

김기호 Ki Ho Kim , 김기수 Ki Soo Kim , 김영희 Young Heui Kim , 고강일 Kang Ii Ko , 박선희 Sun Hee Park , 김진국 Jin Guk Kim , 최현진 Hyun Jin Chio , 고수연 Su Yeon Ko , 배윤주 Yoon-joo Bae , 김용민 Yong-min Kim

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Abstract

Amino acid complex was made with the composition of amino acids quite similar to that of stratum corneum on the skin. In order to evaluate the efficacy of amino acid complex on the skin as an active cosmetic ingredient, we measured cytotoxicity. TIMP-1 expression, moisturizing effect and the amount of horny substance. In cytotoxicity assay, amino acid complex did not show any cytotoxicity. In moisturizing effect test using corneometer, it showed very good moisturizing effect. In TIMP-1 mRNA assay using RT-PCR, moo acid complex showed the increase of TIMP-1 expression, suggesting that amino acid complex have anti-wrinkle effect. Therefore, amino acid complex may be useful as an active ingredient for anti-aging.

Key Words

amino acid, moisturizing, TIMP-1, anti-aging, cosmetic active ingredient

이동렬 Dong Ryul Lee , 강태준 Tae Jun Kang , 김기선 Ki Sun Kim , 이천구 Cheon Koo Lee

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Abstract

The objectives of this study were to find for suitable chemical reagents to use as a green-color protector for Gardenia jasminoldes Green pigment and to compare the effectiveness of these reagents for discoloration prevention. Gardenia jasminoldes Green treated with polyoxyethylene (POE) (12) dimethicone shouted a greener color than those treated with methoxycinnamidopropyl hydroxysultaine ultraviolet stabilizers after ultraviolet radiation (UVR). The experimental results exhibited that the concentration of the POE (12) dimethicone affected the effectiveness of green-color conservation. The greenest rotor was obtained by treating 0.10 wt% aquous solution of Gardenia jasminoldes Green with 1.0 wt% POE (12) dimethicone aqueous solution and the absorbances of the solutions after UVR were increased from 78% to 95% in comparison with the untreated. In addition, the Gardenia jasminoldes Green aqueous solution mixed with POE (12) dimethicone showed great green-color fastness in outdoor weathering exposure. The mechanism of the green color protection under UVR is unclear, however the dimethicone group of POE (12) dimethicone may play and important role in the stabilization.

Key Words

Gardenia jasminoldes green, polyoxyethylene dimethicone, absorbance, green-color conservation

김미례 Mi-rye Kim , 우승은 Seung-eun Woo , 신순오 Sun-oh Shin , 홍성미 Sung-mi Hong , 양시용 Si-yong Yang

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Abstract

Staphylococcus aureus is found on the skin of 78~100% of children and adults with atopic dermatitis (AD) but only on the skin of 2~25% of healthy subjects. It is known that S. aureus and their endotoxins as superantigen have important roles in the exacerbation and prolongation of AD. This study was carried out for the detection of S. aureus in the skin of AD, age, sex, outbreak age of AD, treatment duration, aggravation season, and the relation of ooze and S. aureus. Most patients (84%) with AD show colonization of the skin with S. aureus and there is a correlation between the degree of colonization and the serous exudate. It seems likely that the inhibition of S. aureus is associated with improvement in the skin of AD patients.

Key Words

Staphylococcus aureus, superantigen, atopic dermatitis

김미진 Mi Jin Kim , 정택규 Taek Kyu Jung , 홍인기 In Gi Hong , 윤경섭 Kyung-sup Yoon

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Abstract

Natural essential oils showed anti-microbial activity on relatively broad spectrum of bacterial and fungal species. These materials had much more intensive anti-microbial activities than synthetic preservatives on C. albicans, A niger, and P. acnes, especially. In the experimental group, anti-microbial activity was order of tea tree oil (from Melaleuca alternifolia) > methylparaben > phytoncide (from Chamaecyparis obtusa). Also, natural essential oils had anti-oxidative and anti-inflammatory effects. These results suggest that natural essential oils can be useful as good cosmetic ingredients such as natural preservatives and anti-oxidants.

Key Words

anti-microbial, essential oil, preservative, Melaleuca alternifolia, Chamaecyparis obtusa, anti-oxidative, anti-inflammatory

김경동 Kyung-dong Kim , 나민균 Min-kyun Na , 김상진 Sang-jin Kim

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Abstract

This study was to describe the differences in efficacy and effect of herbal extracts by the part and solvent extraction from the medical plants used as materials of oriental herbs cosmetics. And, this study was to apply to the test method of efficacy and effect related to the antioxidation as herbal extracts, complex of actual ingredient, not existing analytical methods of single ingredient. After screening the medical plants with the antioxidative activity primarily and selecting 11 sorts of medical plants to be used by the part in the literature, this study was to confirm the differences through the well-known test methods like DPPH radical scavenging activity test and hydroxyl radical scavenging activity test. For examples, in case of Trachelospermum asiaticum, compared with the aerial part and fruit, the value of DPPH radical scavenging activity test had 25.2 ± 0.2 and 62.4 ± 1.6 each. It has shown that the value of fruit had 2.4 times higher effect than the one of aerial part. In case of hydroxyl scavenging activity test, it was effective in the fruit, but it has shown that there was no effect on the aerial part. It showed the same phenomena in some other plants. From the result above, this researcher could understand that it needed to consider extracting the medical plants or plants with the active principle by the part. Also, this study was to confirm the differences in effect according to the solvent as it changed the solvent extraction after selecting a plant (Lithospermum erythrorhizon) widely used for medicine and dye. As a result of measuring the actual value of superoxide scavenging activity test, this study was to consider that there were differences by the part or solvent extraction in extracting and using the medical plants as it has shown that the effect differences produced 10~80% according to the solvent. When it was applied to the products, this study has shown that it needed to decrease the possible errors.

Key Words

herbal extracts, cosmetics, medical plants, solvent extraction, antioxidants

이화정 Hwa Jeong Lee , 이충우 Chung Woo Lee , 최명숙 Myoung Suk Choi , 손동주 Dong Ju Son , 홍진태 Jin Tae Hong

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Abstract

Essential oils have been used extensively in pharmacy, medicine, food, beverages, cosmetics, perfumery and aromatherapy. Although anti-bacteria, anti-virus, alleviation of fever operations and an anti-inflammatory properties have been reported, action mechanisms have not been fully discovered. In the present study, anti-inflammatory activities of thirty three essential oils have been evaluated in lipopolysaccharide (LPS)-treated macrophage RAW 264.7 cells by the evaluation of nitric oxide (NO) generation since NO generation is implicated in causal factor of inflammation. Among the tested 33 essential oil, Lemongrass oil showed the most inhibitory effect on LPS-induced NO generation in a dose dependent manner (IC₅₀ : 22 μg/mL). In further study, it was found that Lemongrass oil inhibited the expression of inducible nitric oxide synthase. These results suggest that Lemongrass oil may be useful for improvements of the inflammatory disease such as pimple acne skin.

Key Words

esesential oil, Lemongrass, nitric oxide, RAW 264, iNOS, LPS, RAW 264.7

신현정 Hyun Jung Shin , 김수남 Su Nam Kim , 김정기 Jung Ki Kim , 이병곤 Byeong Gon Lee , 장이섭 Ih Seoup Chang

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Abstract

Although many studies have been performed to elucidate the molecular consequence of factors that regulate skin aging, little is known about the effect of green tea catechins except EGCG. The matrix metalloproteinase (MMP), can degrade matrix proteins and results in a collagen deficiency in photodamaged skin, are known to play an important role in photoaging. This study, investigated the effects of green tea catechins on the UVA-induced MMP-1 expression, activity of MMP-2 and synthesis of type I procollagen in human dermal fibroblasts. We examined eight catechins that naturally exist in green tea leaves and compared their efficacies among them. Most of catechins inhibited the expression of MMP-1 in dose dependent manner, and the levels were reduced, especially, 57.4 and 68.2% by treatment with 1 μM of epigallocatechin-3-gallate (EGCG) and gallocatechin-3-gallate (GCG), respectively. Also, catechins significantly suppressed the activities of MMP-2. Catechins also induced the expression of type I procollagen, however, they acted only at the concentration below 1 μM interestingly. Furthermore, when EGCG:GCG:ECG had the ratio of 0.5:1.5:.1.3, they presented the most effective on procollagen synthesis. Therefore, we concluded that catechins significantly inhibited MMPs and induced collagen synthesis. Taken together, all these results suggested that green tea catechins might be good natural materials act as an anti-photoaging and a skin-aging improving agent.

Key Words

green tea catechin, skin aging, MMP-1, MMP-2, collagen

이희경 Hee Kyung Lee , 백지훈 Ji Hwoon Back , 고재숙 Jae Sook Koh , 박덕훈 Deok Hoon Park , 이종성 Jong Sung Lee , 정은선 Eun Sun Jung

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Abstract

In this study, we evaluated the crow's fret of 95 Korean female by using mechanical assessments; Skin-Visiometer SV 600 and Dermascan C. Transparency profilometry (Skin Visiometer) were using a very thin skin print which allowed parallel light to pass through and was analysed immediately after production. High-frequency (20 MHz) ultrasonography (Dermascan C) enabled non-invasive evaluation in skin thickness and echodensity. We found a correlation between skin roughness and dermal density. Particularly, we showed a significant correlation between skin roughness (R2) and dermal thickness. Also, we demostrated a significant negative correlation between dermal density and dermal thickness (p < 0.05). Therefore, the ultrasonography system might be considered a very useful method in wrinkle evaluation with the transparency profilometry. Further study will be required.

Key Words

wrinkle, skin roughness, dermal intensity, dermal thickness